This is the defaulr cube on Blender 3D.
Delete this cube using keys: Shift+X
Use the keys to add Monkey object: Shift+A
Let’s see the result.
Now will make some tricks
Step 1: use key I and select LocRotScale, see below:
Use the keys R and Z , type 90. This will rotate the object with 90 around Z.
Step 2: change the pozition of frame to next frame, see image below to see where it is.
Repeat all steps 1 and 2. The object will be rotate with 360 degres.
You will have 4 frame for each direction : 0 , 90 , 180 , 270 and 360.
Setup the animation render output like image show below:
Resolution X=100 Y=100.
Frame Range: End Frame: 4.
Output image will be: PNG with RGBA.
The result of animation will be four images. This will be an isometric character sprite